Abstract: The establishment of three-dimensional geological structure model of active fault zones is an important basis for studying active faults, earthquake and geological disasters. Based on the tectonic-stratigraphic entity model flow of the 3D modeling platform SKUA-GOCAD, the UVT and DSI techniques in the geological time domain are used in this study. A 3D geological structure model of the Neogene in the Jiangsu segment of the Tanlu fault system is established, combined with the non-quantified geological foundation and the geological grid function. The three-dimensional model includes fault model and strata model with four layers （the surface, Quaternary bottom, Neogene bottom and bedrock top surface）. The 3D model of Cenozoic erathem in Jiangsu segment of Tanlu fault zone gives the 3D structure and characteristics of Quaternary and Neogene basement quantitatively, and describes the 3D spatial distribution of main active faults. The three-dimensional model can more intuitively understand the Cenozoic stratigraphic units and faults in the studied area, which is of great significance to the in-depth analyses on the sedimentation and structure of active fault zones.